Plasmid

Part:BBa_K4335040:Design

Designed by: Hang Zhang   Group: iGEM22_UESTC-BioTech   (2022-10-10)


pTX2040(Cas9+gRNA+StayGold+ ChlamyHgR)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1690
    Illegal XbaI site found at 1696
    Illegal XbaI site found at 4557
    Illegal PstI site found at 5081
    Illegal PstI site found at 5154
    Illegal PstI site found at 5807
    Illegal PstI site found at 6353
    Illegal PstI site found at 7775
    Illegal PstI site found at 7979
    Illegal PstI site found at 8009
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1690
    Illegal NheI site found at 5422
    Illegal NheI site found at 13119
    Illegal PstI site found at 5081
    Illegal PstI site found at 5154
    Illegal PstI site found at 5807
    Illegal PstI site found at 6353
    Illegal PstI site found at 7775
    Illegal PstI site found at 7979
    Illegal PstI site found at 8009
    Illegal NotI site found at 4747
    Illegal NotI site found at 10994
    Illegal NotI site found at 12526
    Illegal NotI site found at 13816
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1690
    Illegal BglII site found at 4551
    Illegal BglII site found at 5814
    Illegal BglII site found at 6609
    Illegal BglII site found at 7299
    Illegal BglII site found at 9378
    Illegal BamHI site found at 1684
    Illegal BamHI site found at 3498
    Illegal BamHI site found at 9719
    Illegal XhoI site found at 2858
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1690
    Illegal XbaI site found at 1696
    Illegal XbaI site found at 4557
    Illegal PstI site found at 5081
    Illegal PstI site found at 5154
    Illegal PstI site found at 5807
    Illegal PstI site found at 6353
    Illegal PstI site found at 7775
    Illegal PstI site found at 7979
    Illegal PstI site found at 8009
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1690
    Illegal XbaI site found at 1696
    Illegal XbaI site found at 4557
    Illegal PstI site found at 5081
    Illegal PstI site found at 5154
    Illegal PstI site found at 5807
    Illegal PstI site found at 6353
    Illegal PstI site found at 7775
    Illegal PstI site found at 7979
    Illegal PstI site found at 8009
    Illegal NgoMIV site found at 1425
    Illegal NgoMIV site found at 1963
    Illegal NgoMIV site found at 2071
    Illegal NgoMIV site found at 3613
    Illegal NgoMIV site found at 5323
    Illegal NgoMIV site found at 6641
    Illegal NgoMIV site found at 10439
    Illegal AgeI site found at 13613
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4670
    Illegal BsaI.rc site found at 4652
    Illegal SapI.rc site found at 9034


Design Notes

Source

References